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Novel regulators of +TIP localisation and function

PGR-P-64

Key facts

Type of research degree
PhD
Application deadline
Ongoing deadline
Country eligibility
International (open to all nationalities, including the UK)
Funding
Non-funded
Additional supervisors
Dr Ewan Morrison, Dr Sandra Bell
Schools
School of Medicine
Research groups/institutes
Leeds Institute of Medical Research at St James's
<h2 class="heading hide-accessible">Summary</h2>

Microtubules (MTs) are a key cytoskeletal network in all eukaryotic cells. MTs grow and shrink primarily through the addition or loss of tubulin heterodimers from their plus end. The +TIPs are a class of proteins that specifically localize to the growing plus end of MTs. They play important roles in most MT-dependent processes, including mitosis, migration and differentiation. As such they have been implicated in a range of human diseases and developmental disorders and could represent novel therapeutic targets. EB proteins (EB1, EB2 and EB3) form the core of many +TIP complexes, directly contacting the growing MT end and acting as a scaffold that allows other +TIPs to localize to this site. Given the key role of EB proteins in +TIP assemblies, there is great interest in defining how their localisation is regulated. We performed a small inhibitory RNA (siRNA) screen to identify genes that regulate EB1 levels at MT tips. This identified a number of hits that had not previously been implicated in EB protein function. The aim of this project is to validate these hits and functionally characterise the role of selected proteins in regulating both EB protein activity and MT function in appropriate cellular models. The work will involve cell biological and biochemical studies, giving exposure to a variety of techniques including cell culture and advanced cell imaging methods. Environment: The student will be based in the University of Leeds in the section of Ophthalmology and Neuroscience (OPNE), School of Medicine, University Of Leeds. The appointed student will have the opportunity to learn a broad range of techniques described above. For further details of the Institute&rsquo;s research visit https://medicinehealth.leeds.ac.uk/homepage/160/leeds_institute_of_medical_research_at_st_jamess

<h2 class="heading">How to apply</h2>

<p>Applications can be made at any time. To apply for this project applicants should complete a<a href="https://medicinehealth.leeds.ac.uk/downloads/download/129/faculty_graduate_school_-_application_form"> Faculty Application Form</a> and send this alongside a full academic CV, degree transcripts (or marks so far if still studying) and degree certificates to the Faculty Graduate School <a href="mailto:fmhpgradmissions@leeds.ac.uk">fmhpgradmissions@leeds.ac.uk</a>.</p> <p>We also require 2 academic references to support your application. Please ask your referees to send these <a href="https://medicinehealth.leeds.ac.uk/downloads/download/130/faculty_graduate_school_-_scholarship_reference_form">references</a> on your behalf, directly to<a href="mailto:fmhpgradmissions@leeds.ac.uk">fmhpgradmissions@leeds.ac.uk</a></p> <p>If you have already applied for other projects using the Faculty Application Form this academic session you do not need to complete this form again. Instead you should email <a href="mailto:fmhpgradmissions@leeds.ac.uk">fmhpgradmissions@leeds.ac.uk</a> to inform us you would like to be considered for this project.</p> <p>If English is not your first language, you must provide evidence that you meet the University&#39;s minimum English language requirements (below).</p> <p><em>We welcome applications from all suitably-qualified candidates, but UK black and minority ethnic (BME) researchers are currently under-represented in our Postgraduate Research community, and we would therefore particularly encourage applications from UK BME candidates. All scholarships will be awarded on the basis of merit.</em></p>

<h2 class="heading heading--sm">Entry requirements</h2>

A degree in biological sciences, dentistry, medicine, midwifery, nursing, psychology or a good honours degree in a subject relevant to the research topic. A Masters degree in a relevant subject may also be required in some areas of the Faculty. For entry requirements for all other research degrees we offer, please contact us.

<h2 class="heading heading--sm">English language requirements</h2>

Applicants whose first language is not English must provide evidence that their English language is sufficient to meet the specific demands of their study. The Faculty of Medicine and Health minimum requirements in IELTS and TOEFL tests for PhD, MSc, MPhil, MD are: &acirc;&euro;&cent; British Council IELTS - score of 6.5 overall, with no element less than 6.0 &acirc;&euro;&cent; TOEFL iBT - overall score of 92 with the listening and reading element no less than 21, writing element no less than 22 and the speaking element no less than 23.

<h2 class="heading">Contact details</h2>

<p>For further information please contact the Graduate School Office<br /> e: <a href="mailto:fmhpgradmissions@leeds.ac.uk">fmhpgradmissions@leeds.ac.uk</a>, t: +44 (0)113 343 8221.</p>