Visualising Conformational Dynamics of Proteins and Biomolecular Assemblies with High-Speed AFM

Proteins and other biomolecules are highly dynamic, undergoing conformational changes, oligomerisation, and transient interactions that are essential for biological function. Understanding these dynamics in near-native environments remains a major challenge. This project will use high-speed atomic force microscopy (HS-AFM) to capture the real-time structural dynamics of proteins and molecular assemblies at nanometre and sub-second resolution. Students will optimise experimental systems to visualise proteins in near-native environments and quantify their conformational dynamics using advanced image analysis and computational tools. Integrating HS-AFM data with complementary structural techniques (cryo-EM, X-ray crystallography) and computational approaches (molecular dynamics simulations) will allow a holistic view of protein conformational landscapes, interaction networks, and cooperative behaviour.<br /> <br /> The project is broadly applicable to a range of biomolecular systems, from ion channels and transporters to soluble protein complexes and DNA assemblies, enabling mechanistic insights into fundamental biological processes and disease-relevant protein function.<br />

<p style="text-align:start; margin-bottom:16px">Initially, please contact <a href="mailto:g.r.heath@leeds.ac.uk?subject=PhD%20project%3A%20Visualising%20Conformational%20Dynamics%20of%20Proteins%20and%20Biomolecular%20Assemblies%20with%20High-Speed%20AFM">Dr George Heath</a> (<a href="mailto:g.r.heath@leeds.ac.uk">g.r.heath@leeds.ac.uk</a>), formal applications for research degree study should be made online through the <a href="https://www.leeds.ac.uk/research-applying/doc/applying-research-degrees">University's website</a>. Please state clearly in the planned Course of Study section that you are applying for <em><strong>PHD Physics & Astronomy FT</strong></em>, in the research information section that the research degree you wish to be considered for is <em><strong>Visualising Conformational Dynamics of Proteins and Biomolecular Assemblies with High-Speed AFM</strong></em> as well as <a href="https://eps.leeds.ac.uk/physics/staff/6132/dr-george-heath">Dr George Heath</a> as your proposed supervisor <em><strong>and in the finance section, please state clearly the funding that you are applying for, if you are self-funding or externally sponsored.</strong></em></p> <p>Applications will be considered on an ongoing basis.  Potential applicants are strongly encouraged to contact the supervisors for an informal discussion before making a formal application.  We also advise that you apply at the earliest opportunity as the application and selection process may close early, should we receive a sufficient number of applications or that a suitable candidate is appointed.</p> <p>If you are applying for University or School Scholarships for 2026/27 entry, with external sponsorship or you are funding your own study, please ensure you provide your supporting documents at the point you submit your application:</p> <ul> <li>Full Transcripts of all degree study or if in final year of study, full transcripts to date including the grading scheme</li> <li>Personal Statement outlining your interest in the project</li> <li>CV</li> </ul> <p>If English is not your first language, you must provide evidence that you meet the University's minimum English language requirements (below).</p> <p><em>As an international research-intensive university, we welcome students from all walks of life and from across the world. We foster an inclusive environment where all can flourish and prosper, and we are proud of our strong commitment to student education. Across all Faculties we are dedicated to diversifying our community and we welcome the unique contributions that individuals can bring, and particularly encourage applications from, but not limited to Black, Asian, people who belong to a minority ethnic community, people who identify as LGBT+ and people with disabilities. Applicants will always be selected based on merit and ability.</em></p>

Applicants to research degree programmes should normally have at least a first class or an upper second class British Bachelors Honours degree (or equivalent) in an appropriate discipline. The criteria for entry for some research degrees may be higher, for example, several faculties, also require a Masters degree. Applicants are advised to check with the relevant School prior to making an application. Applicants who are uncertain about the requirements for a particular research degree are advised to contact the School or Graduate School prior to making an application.

The minimum English language entry requirement for research postgraduate research study is an IELTS of 6.0 overall with at least 5.5 in each component (reading, writing, listening and speaking) or equivalent. The test must be dated within two years of the start date of the course in order to be valid. Some schools and faculties have a higher requirement.

<p style="text-align:start; margin-bottom:11px"><strong>Self-Funded or externally sponsored students are welcome to apply.</strong></p> <p><strong>Scholarship opportunities open from October 2025</strong></p> <p><strong>UK</strong> – The <a href="https://phd.leeds.ac.uk/funding/209-leeds-doctoral-scholarships-2024">Leeds Doctoral Scholarship</a> <strong>(closing date: 1 April 2026)</strong> and <a href="https://phd.leeds.ac.uk/funding/234-leeds-opportunity-research-scholarship-2022">Leeds Opportunity Research Scholarship</a> <strong>(closing date: 1 April 2026)</strong> are available to UK applicants.  <a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a> is available to graduates of the University of Leeds.</p> <p><strong>Non-UK</strong> – The <a href="https://phd.leeds.ac.uk/funding/48-china-scholarship-council-university-of-leeds-scholarships-2021">China Scholarship Council - University of Leeds Scholarship</a> is available to nationals of China <strong>(closing date: 7 January 2026)</strong>. <a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a> is available to graduates of the University of Leeds.</p> <p>Please note that if you are successful in securing an academic offer for PhD study, this does not mean that you have been successful in securing an offer of funding.</p> <p>If you are applying for the Leeds Doctoral Scholarship, Leeds Opportunity Research Scholarship, China Scholarship Council-University of Leeds Scholarship, you will need to complete a separate application, specific to these scholarships, to be considered for funding.</p> <p>You will be responsible for paying the overtime fee in full in your writing up/overtime year (£340 in Session 2025/26), but the scholarship maintenance allowance will continue to be paid for up to 6 months in the final year of award.</p> <p>Important: Please note that that the award does not cover the costs associated with moving to the UK.  All such costs (<a href="https://www.leeds.ac.uk/international-visas-immigration/doc/applying-student-visa">visa, Immigration Health Surcharge</a>, flights etc) would have to be met by yourself, or you will need to find an alternative funding source. </p> <p>Please refer to the <a href="https://www.ukcisa.org.uk/">UKCISA</a> website for information regarding Fee Status for Non-UK Nationals.</p>

<p style="text-align:start">For further information about your application, please contact PGR Admissions by email to <a href="mailto:phd@engineering.leeds.ac.uk?subject=PhD%20Project%3A%20Visualising%20Conformational%20Dynamics%20of%20Proteins%20and%20Biomolecular%20Assemblies%20with%20High-Speed%20AFM%20(Dr.%20George%20Heath)">phd@engineering.leeds.ac.uk</a></p> <p>For further information about this project, please contact Dr George Heath by email: <a href="mailto:g.r.heath@leeds.ac.uk?subject=PhD%20Project%3A%20Visualising%20Conformational%20Dynamics%20of%20Proteins%20and%20Biomolecular%20Assemblies%20with%20High-Speed%20AFM">g.r.heath@leeds.ac.uk</a></p>