Filming photosynthesis: using molecular dynamics simulations and fluorescence experiments to assess the dynamics of light harvesting proteins

The plant protein Light-Harvesting Complex II (LHCII) allows efficient absorption of solar energy and is essential for photosynthesis. LHCII is one of the most abundant membrane proteins on Earth, found in high numbers in the chloroplasts of all green plants. LHCII proteins undergo dynamic structural changes which alter its capacity for light harvesting but our understanding of how LHCII functions at the molecular level is often based on a static picture. Understanding the dynamics of LHCII is important because this molecular information supports the development of improved crops and novel solar nanotechnologies. <br /> <br /> In this project, we will use an interdisciplinary approach that combines computer simulations termed &ldquo;molecular dynamics&rdquo; together with fluorescence spectroscopy and fluorescence microscopy experiments to: (1) determine the effect of LHCII-LHCII interactions on its conformational switching, (2) study how LHCII interacts with other proteins cofactors proposed to modulate its action, and (3) quantify the effect of lipid composition on the protein stability and protein-protein interactions. This project will provide novel insights into the different conformations which are known to modulate the state of LHCII underlying its critical function of capturing solar energy. <br /> <br /> This project would suit a student interested in biochemistry, chemistry or physics and willing to learn about computational biophysics.

<p>Formal applications for research degree study should be made online through the&nbsp;<a href="https://www.leeds.ac.uk/research-applying/doc/applying-research-degrees" rel="noreferrer noopener" target="_blank">University&#39;s website</a>. Please state clearly in the Planned Course of Study section that you are applying for&nbsp;<strong><em>PhD Physics &amp; Astronomy FT,</em></strong>&nbsp;in the research information section&nbsp;that the research project you wish to be considered for is <em><strong>Filming photosynthesis: using molecular dynamics simulations and fluorescence experiments to assess the dynamics of light harvesting proteins</strong></em>&nbsp;as well as&nbsp;<a href="https://eps.leeds.ac.uk/physics/staff/4098/dr-peter-adams" rel="noreferrer noopener" target="_blank">Dr Peter Adams</a>&nbsp;and&nbsp;<a href="https://medicinehealth.leeds.ac.uk/medicine/staff/485/dr-antreas-kalli" rel="noreferrer noopener" target="_blank">Dr Antreas Kalli</a>&nbsp;as your proposed supervisors&nbsp;and in the finance section, please state clearly&nbsp;<em><strong>the funding that you are applying for, if you are self-funding or externally sponsored</strong></em>.&nbsp;</p> <p>If English is not your first language, you must provide evidence that you meet the University&#39;s minimum English language requirements (below).</p> <p><em>As an international research-intensive university, we welcome students from all walks of life and from across the world. We foster an inclusive environment where all can flourish and prosper, and we are proud of our strong commitment to student education. Across all Faculties we are dedicated to diversifying our community and we welcome the unique contributions that individuals can bring, and particularly encourage applications from, but not limited to Black, Asian, people who belong to a minority ethnic community, people who identify as LGBT+ and people with disabilities. Applicants will always be selected based on merit and ability.</em></p> <p class="MsoNoSpacing">Applications will be considered on an ongoing basis. &nbsp;Potential applicants are strongly encouraged to contact the supervisors for an informal discussion before making a formal application. We also advise that you apply at the earliest opportunity as the application and selection process may close early, should we receive a sufficient number of applications or that a suitable candidate is appointed.</p> <p>Please note that you must provide the following documents in support of your application by the closing date of 3 April 2024 for Leeds Opportunity Research Scholarship or&nbsp;8 April 2024 for Leeds Doctoral Scholarship:</p> <ul> <li>Full Transcripts of all degree study or if in final year of study, full transcripts to date</li> <li>Personal Statement outlining your interest in the project</li> <li>CV</li> </ul>

Applicants to research degree programmes should normally have at least a first class or an upper second class British Bachelors Honours degree (or equivalent) in an appropriate discipline. The criteria for entry for some research degrees may be higher, for example, several faculties, also require a Masters degree. Applicants are advised to check with the relevant School prior to making an application. Applicants who are uncertain about the requirements for a particular research degree are advised to contact the School or Graduate School prior to making an application.

The minimum English language entry requirement for research postgraduate research study is an IELTS of 6.0 overall with at least 5.5 in each component (reading, writing, listening and speaking) or equivalent. The test must be dated within two years of the start date of the course in order to be valid. Some schools and faculties have a higher requirement.

<p lang="EN-US" xml:lang="EN-US"><strong>UK&nbsp;</strong>&ndash; the&nbsp;<a href="https://phd.leeds.ac.uk/funding/209-leeds-doctoral-scholarships-2024">Leeds Doctoral Scholarship Award</a>&nbsp;paying Academic Fees and Maintenance matching UKRI rate of &pound;18,622&nbsp;(for 23/24) per year for 3.5 years;&nbsp;<a href="https://phd.leeds.ac.uk/funding/234-leeds-opportunity-research-scholarship-2024">Leeds Opportunity Scholarships</a>&nbsp;(for applicants from Black, Asian and other minoritised ethnic groups) paying Academic Fees and Maintenance UKRI rate of &pound;18,622 per year for 3.5 years; the&nbsp;<a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a>&nbsp;is available to previous University of Leeds graduates offering 10% discount on Academic Fees. Please contact the supervisors for advice on applying.&nbsp;&nbsp;</p> <p lang="EN-US" xml:lang="EN-US"><strong>International Students</strong>&nbsp;&ndash; The&nbsp;<a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a>&nbsp;is available to previous University of Leeds graduates offering 10% discount on Academic Fees. Please contact the supervisors for advice on applying.&nbsp;&nbsp;</p> <p lang="EN-US" xml:lang="EN-US"><strong>Self-funded students and externally sponsored students (e.g., government scholarships) are also welcome to apply.&nbsp;&nbsp;</strong></p>

<p lang="EN-US" xml:lang="EN-US">For further information about this project, please contact Dr Peter Adams by email to&nbsp;<a href="mailto:EMAIL@leeds.ac.uk" rel="noreferrer noopener" target="_blank">p.g.adams@leeds.ac.uk</a>&nbsp;or by telephone to&nbsp;+44 (0)113 343 9718.&nbsp;</p> <p lang="EN-US" xml:lang="EN-US">For further information about your application, please contact Doctoral College Admissions by email to&nbsp;<a href="mailto:maps.pgr.admissions@leeds.ac.uk" rel="noreferrer noopener" target="_blank">maps.pgr.admissions@leeds.ac.uk</a>&nbsp;</p>