Revealing the mechanism of photoprotection in light-harvesting proteins using novel nanotechnology and advanced time-resolved fluorescence microscopy

The Light-Harvesting Complex II (LHCII) is the major antenna protein found in green plants and is essential for efficient photosynthesis. It has a crucial role in absorbing solar photons and channelling this energy to the next part of the system and also a secondary role in protecting the biological organism from high intensity sunlight by safely dissipating excess energy. However, the molecular mechanism for the process of photoprotective energy dissipation within LHCII is highly debated and varies between different organisms. This project will use our latest developments in membrane nanotechnology and world-class microscopy and spectroscopy to advance our understanding of the true nature of photoprotection in LHCII.<br /> <br /> The laboratory in Leeds recently developed a new technique to control the organization of membrane proteins within a model lipid bilayer by applying electric fields. The proteins can be visualized by a specialized fluorescence microscope that also acquires fluorescence lifetimes to measure the energetic state of the LHCII. This combination of techniques provides a new way to assess protein-protein interactions and correlate them to possibility of energy dissipation in LHCII. This project will compare a systematic series of samples with these techniques: (i) comparison of different LH proteins from plants versus bacteria versus algae, (ii) effect of pH and other parameters which are known to trigger photoprotection, (iii) effect of key modulator proteins with LHCII that are known to modulate photoprotection (e.g., PsbS). The outcome of these investigations will be a quantitative model for how various LH proteins can trigger photoprotection. This project will provide a major advance in our understanding of photosynthesis with wider relevance to bioenergy and agriculture.<br />

<p>Formal applications for research degree study should be made online through the&nbsp;<a href="https://www.leeds.ac.uk/research-applying/doc/applying-research-degrees">University&#39;s website</a>. Please state clearly in the Planned Course of Study section that you are applying for<em><strong> PHD Physics &amp; Astronomy FT</strong></em> and in the research information section&nbsp;that the research degree you wish to be considered for is <em><strong>Revealing the mechanism of photoprotection in light-harvesting proteins using novel nanotechnology and advanced time-resolved fluorescence microscopy</strong></em>&nbsp;as well as&nbsp;<a href="https://eps.leeds.ac.uk/physics/staff/4098/dr-peter-adams">Dr Peter Adams</a>&nbsp;and <a href="https://eps.leeds.ac.uk/physics/staff/4107/professor-stephen-evans">Prof. Stephen Evans</a> as your proposed supervisors.</p> <p>If English is not your first language, you must provide evidence that you meet the University&#39;s minimum English language requirements (below).</p> <p><em>As an international research-intensive university, we welcome students from all walks of life and from across the world. We foster an inclusive environment where all can flourish and prosper, and we are proud of our strong commitment to student education. Across all Faculties we are dedicated to diversifying our community and we welcome the unique contributions that individuals can bring, and particularly encourage applications from, but not limited to Black, Asian, people who belong to a minority ethnic community, people who identify as LGBT+ and people with disabilities. Applicants will always be selected based on merit and ability.</em></p> <p class="MsoNoSpacing">Applications will be considered on an ongoing basis. &nbsp;Potential applicants are strongly encouraged to contact the supervisors for an informal discussion before making a formal application. &nbsp;We also advise that you apply at the earliest opportunity as the application and selection process may close early, should we receive a sufficient number of applications or that a suitable candidate is appointed.</p> <p>Please note that you must provide the following documents at the point you submit your application:</p> <ul> <li>Full Transcripts of all degree study or if in final year of study, full transcripts to date</li> <li>Personal Statement outlining your interest in the project</li> <li>CV</li> <li>Funding information including any alternative sources of funding that you are applying for or if you are able to pay your own fees and maintenance</li> </ul>

Applicants to research degree programmes should normally have at least a first class or an upper second class British Bachelors Honours degree (or equivalent) in an appropriate discipline. The criteria for entry for some research degrees may be higher, for example, several faculties, also require a Masters degree. Applicants are advised to check with the relevant School prior to making an application. Applicants who are uncertain about the requirements for a particular research degree are advised to contact the School or Graduate School prior to making an application.

The minimum English language entry requirement for research postgraduate research study is an IELTS of 6.0 overall with at least 5.5 in each component (reading, writing, listening and speaking) or equivalent. The test must be dated within two years of the start date of the course in order to be valid. Some schools and faculties have a higher requirement.

<p style="margin-bottom:11px"><strong>Self-funded students and externally sponsored students are also very welcome to apply.</strong></p> <p style="margin-bottom:11px"><strong>UK</strong> &ndash; the&nbsp;<a href="http://phd.leeds.ac.uk/funding/209-leeds-doctoral-scholarships-2022">Leeds Doctoral Scholarship Award</a> paying Academic Fees and Maintenance matching UKRI rate of &pound;17,668 per year for 3.5 years; <a href="https://phd.leeds.ac.uk/funding/234-leeds-opportunity-research-scholarship-2023">Leeds Opportunity Scholarships</a> paying Academic Fees and Maintenance UKRI rate of &pound;17,668 per year for 3.5 years (available to Black and Asian applicants); the&nbsp;<a href="https://phd.leeds.ac.uk/funding/102-bell-burnell-scholarship-award-awarded-by-the-iop">Bell Burnell Scholarship Award</a> paying maintenance and Academic Fees for up to 4 years to applicants who are from groups that are currently under-represented in Physics (Awarded by the IOP); the&nbsp;<a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a> is available to previous University of Leeds graduates offering 10% discount on Academic Fees. Please contact the supervisors for advice on applying.</p> <p><strong>International Students</strong>&nbsp;&ndash;&nbsp;<a href="https://phd.leeds.ac.uk/funding/48-china-scholarship-council-university-of-leeds-scholarships-2023">China Scholarship Council-University of Leeds Scholarship Award</a> paying maintenance and Academic Fees for 3 years; the&nbsp;<a href="https://phd.leeds.ac.uk/funding/102-bell-burnell-scholarship-award-awarded-by-the-iop">Bell Burnell Scholarship Award</a> paying maintenance and Academic Fees for up to 4 years to applicants who are from groups that are currently under-represented in Physics (Awarded by the IOP); the&nbsp;<a href="https://phd.leeds.ac.uk/funding/60-alumni-bursary">Alumni Bursary</a> is available to previous University of Leeds graduates offering 10% discount on Academic Fees. Please contact the supervisor for advice on applying.</p> <p class="MsoNoSpacing"><strong>Important:&nbsp;</strong> Any costs associated with your arrival at the University of Leeds to start your PhD including flights, immigration health surcharge/medical insurance and Visa costs are not covered under this studentship.</p> <p>Please refer to the <a href="https://www.ukcisa.org.uk/">UKCISA</a> website for information regarding Fee Status for Non-UK Nationals.</p>

<p>For further information about this project, please contact Dr Peter Adams:&nbsp;e:&nbsp;<a href="mailto:EMAIL@leeds.ac.uk">p.g.adams@leeds.ac.uk</a>, t: +44 (0)113 343 9718.</p> <p>For further information about your application, please contact Doctoral College Admissions: e:&nbsp;<a href="mailto:maps.pgr.admissions@leeds.ac.uk">maps.pgr.admissions@leeds.ac.uk</a></p>